ABSTRACT
Objective To develop grooming behavioral ethogram of fruitfly Drosophila melanogaster and to study its behavioral sequence.Methods Grooming behavior of fruitfly was observed by focal sampling and scan sampling,and the data analysis was carried out by software Ethom.Results A total of 15 types of grooming behavior were displayed by fruitfly,with no significant differences between genders.The front legs have significantly more transitions to front legs,and significantly less to wings and rear legs,similarly,the rear legs have significantly high transitions to rear legs and wings,but a significantly reduced transition to front legs.(Conclusion)The grooming behaviors of fruitfly Drosophila melanogaster are sterreotype behaviors with specific behavior sequences.
ABSTRACT
Objective To develop a method to analyze functional grouping cDNA microarray gene expression in microvessels and neurons from rat brain using laser capture microdissection(LCM). Methods Microvessels and neurons were captured using the PixCell ⅡLCM instrument. The total RNA was extracted from the LCM samples according to the manufacturer's protocol of RNAqueous-Micro kit. The total RNA were processed for one round of T7-based RNA amplification; cDNA probe were synthesized using gene-specific primer and labeled for cDNA microarray analysis. Results Amplified RNA from microvessels and neurons allowed us to measure 96 gene expression in functional grouping cDNA microarray. Conclusion LCM allowed us to harvest pure microvessels and neurons from their native tissue environment, combined with methods of T7 RNA amplification and gene-specific primer amplification so that we can analyze functional grouping cDNA profiling in LCM samples.